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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Eur J Oral Sci. 2011 Dec;119(Suppl 1):329–337. doi: 10.1111/j.1600-0722.2011.00860.x

Fig. 2.

Fig. 2

Apoptosis [determined using terminal deoxynucleotidyl transferase (TdT)-mediated biotin–dUTP nick-end labelling (TUNEL)] in day-7 mouse maxillary first molars from wild-type (A, B), enamelin (Enam) heterozygous (C, D), and Enam null (E, F) mice. Arrowheads in panels A and C indicate trace apoptotic staining in the molars from wild-type and Enam heterozygous mice. Panels on the right (B, D, F) detail the ameloblast layer of the central cusp tip. In panel E the arrowheads mark a mineralized nodule associated with apoptotic nuclei. Light brown staining of the extracellular enamel matrix in the Enam heterozygote is background. Arrows indicate a cyst-like expansion not associated with apoptotic nuclei. Bars, panels on left = 200 µm; panels on right = 100 µm. Ameloblasts near the cusp tips are in the maturation stage; ameloblasts nearer the cervical loop are in the secretory stage.