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. 2012 Jan 11;32(2):490–505. doi: 10.1523/JNEUROSCI.4382-11.2012

Figure 6.

Figure 6.

Stalled motor neuron migration results in bucking of the ventral Pax6 domain following γ-catenin(L127A) expression. A–C, Lhx1-expressing ventral interneurons and LMCl neurons (B, C, left-pointing arrowheads) and islet-1 cells (I) are found close to the ventricle (A, B, dotted lines). Electroporated neurons are marked by β-gal immunofluorescence (A, green). B, C, Right-pointing arrowheads, Absence of effect on dorsal interneuron populations. D, E, The ventral Pax 6 (green) progenitor domain buckles following γ-catenin(L127A) expression but the Nkx2.2 (red) domain is unperturbed. Electroporation is marked by β-gal immunofluorescence (D, blue). D, Dotted lines, Ventricle surfaces. E, Magnified image of the ventral progenitor domains of the right side of the spinal cord in D. Note that the electroporated cells (stalled LMC neurons) are close to the ventricle, whereas the majority of the buckled ventral Pax6 domain is not electroporated. F, Lhx1 immunofluorescence focusing on the ventricular zone of the adjacent section to that shown in D and E. F′, Position of the Lhx1 cells (outlined by the dotted lines) in relation to the Pax6 domain of E. G–I, Transitin immunofluorescence (G, H) following electroporation of γ-catenin(L127A). The right side of the spinal cord in G was electroporated. H, I, Magnified images of the dotted box in G. H, Dotted lines, Ventricle surfaces. I, β-Gal immunoreactivity showing electroporated cells. J–L, β-Catenin (β-cat; J, K) immunofluorescence in radial glia in the ventral horn at stage 25 after γ(L127A) expression marked by β-gal immunoreactivity in J and L. M, N, Islet-1 (green) and Pax6 (red) immunofluorescence at stage 26 following γ-catenin(L127A) expression, showing that motor neurons stalled in their migration are found adjacent to the bucked Pax6 domain (as in F, F′). Motor neuron position relative to the Pax6 domain is therefore normal. β-gal immunoreactivity (N, blue) shows electroporated neurons. O, Summary of the data in this figure showing the relative positions of transitin radial glia, progenitor domains, and LMC neurons following γ-catenin(L127A) expression.