Figure 9.

Downregulation of canonical Wnt signaling does not disrupt LMC neuron organization. A–D, Lack of effect on LMC organization of expression of wild-type GSK3β. A, Schematic of action of the construct. B–D, HA-tag (B, C, green), Islet-1 (B, red; D, green) and Lhx1 (C, D, red) immunoreactivity following electroporation of the construct. E–L, Downregulation of Wnt signaling pathway by expression of constitutively active GSK3β construct (E–H) or dominant-negative TCF transcription factor expression (I–L). E, I, Summary of action of each construct. Islet-1(2) immunohistochemistry (F, G, J, K) reveals normal motor neuron migration and divisional segregation following expression of the constructs, marked by HA immunoreactivity (F, H, J, L). Islet1(2) immunofluorescence is lower in LMCl than LMCm.