Table 2. Sequenced clones from SSH of OS/NWL thyroid cDNA (3 days old).
| Clone | Length (bp) | Specificity of hybridization |
Significant alignment | Accession number |
|---|---|---|---|---|
| 1.2.1. | 385 | No | Chicken EST | AF370360 |
| Human coatomer protein | U24105 | |||
| 1.2.2. | 378 | Thyroid specific | None | AJ414704 |
| 1.1.M3 | 400 | No | None | AJ414705 |
| 1.1.V5 | 505 | Thyroid specific | Human thyroglobulin | X05615 |
| 2.2.6E | 904 | OS specific | ALV ev–6 | AY013305.1 |
| ALV ev–3 | AY013304.1 | |||
| ALV ev–1 | AY013303.1 | |||
| ALV strain NTRE–2 | MI4897.1 | |||
| 3.2.2.A | 514 | OS specific | ALV ev–3 | AY013304.1 |
| ALV ev–1 | AY013303.1 | |||
| 6.2.1.E2 | 854 | Thyroid specific | unknown gene | AJ414706 |
| 6.2.2.C3 | 620 | OS specific (quantitative) |
None | AJ414707 |
| CB8G | 400 | Thyroid specific | Human thyroglobulin | X05615 |
Suppression substractive hybridization of RNA prepared from thyroid glands of OS and normal control (inbred B12 B12 line) chicks at the age of 3 days, i.e. before onset of lymphoid infiltration. Sequences were analysed by BLAST search (National Center for Biotechnology Information). EST = expressed sequence tag, ALV = avian leucosis virus, ev = endogenous virus (adapted from Vasicek et al., 2001)