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. 2012 Jan 27;5:76. doi: 10.1186/1756-0500-5-76

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Copyright ©2012 Sanou et al; BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Interleukin-10 (IL-10) secreting T cells Determination. IL-10 is a cytokine with anti-inflammatory properties, has a central role in infection by limiting the immune response to pathogens. CD4+CD25+ producing Interleukin-10 were determined by gating on CD4 T cells on Light scatter and using isotype control (a). CD25 secreting IL10 were identified in all CD4 producing IL10 (b) and also in CD4+CD25+ T cells (c) to access proportion in all CD4 producing Interleukine -10 and proportion of those produce the cytokine among all CD25 subset.