Figure 1.

Deficiencies in motor coordination and hindlimb reflex in Chd4 mutant mice. A, Immunohistochemistry of Chd4 (red) in the sciatic nerves of control and conditional knock-out mice at P8. Cell nuclei were counterstained with Hoechst (blue). Scale bar, 30 μm. B, Normal hindlimb postural reflex in a wild-type mouse (Chd4 f/f) characterized by spreading of the limbs. Abnormal reflex in Chd4 mutant mouse where hindlimbs are crossed and withdrawn (P55). C, Gross examination of sciatic nerve dissected from Chd4 KO and control mice at P30. D, RotaRod performance measuring latency to fall for 4-week-old mice. Three trials per session were measured with 20 min interval between trials. Statistical significance was determined using Student's t test: *p < 0.01; **p < 0.001. Error bars indicate SD.