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Persistence of Schwann cell proliferation. A–D, BrdU immunohistochemical analysis of P8 Chd4 cko sciatic nerve demonstrates an increased number of proliferating cells compared with control littermates. The BrdU-positive nuclei were counted, and proliferation was determined as a percentage of BrdU-positive nuclei compared with all Hoechst-stained nuclei in the nerve section. Scale bar, 30 μm. p < 0.019; n = 3 for each genotype.
