Table 3. Primers used for analysis of chromosome 1.

Marker	Annealing temperature, °C	Primer sequence, 5'→ 3'	Position on 1st chromosome, Mb	Expected length of PCR fragment, bp
D1Rat150	64	AGAGGCAATGAAGTCCCTGA ATCCAGTGTCAACCTTTGGC	15.8	238
D1Rat234	62	GGGTACACTGGACTGGGAAA GCTGCCATTTAGTCTGGCTT	41.2	152
D1Rat196	62	TGCTTTTCAGATTCTCCTCC TACTGAGTCAATTTCTCTTGG	55.1	177
D1Rat224	58-64	AAAGCAATCTGTTTAAAAACAGTCA GCGTTTTCTCTGTCGCAATT	90.3	167
D1Rat30	64	AATTTCTGTCCCACATTTCCC TTCCAGGGACAAGCTACCTG	100.6	223
D1Rat183	64	CAGAAGCAAGCACACCAGTC TGTATTGGCTGGGAAGTTGG	131.2	226
D1Rat131	64	TCTGCGACTACCTTGGGTTT CCAGCAATTGAAATAACATTTTCA	145.7	176
D1Rat54	64	CTGACGGAAAAAAGGACAGG GTCTGCCTGCTGGGATTAAG	168.0	173
D1Rat219	60-58	GGAAGGGATCACATTGCATT GCAAAAGGACCTGTTGAAGC	188.0	248
D1Rat287	62-60	GTGCTATGGTGGGCAAGTTT GGGCGTGACCAGGTTACTTA	190.3	211
D1Rat168	64	AAGGAGCCACTAACTGTTCCC TCTCCAAAGCGGCTGAGTAT	204.8	210
D1Rat117	64	CCATGAGTTGCCATGGCTAT AATGCCACACAGAGAAGGGT	219.8	125
D1Rat76	64	GAAAGAGTGTGCGTGTGCAT CTTCTGTCTCCTCGCCAATC	230.6	144
D1Rat81	64	TGGTTCCAATGGATACCCATA TGAAGACTGAATCCCCCAAC	250.4	169
D1Rat86	64	AATACATGATGCTGTGGATTGG ACCCATTCCCACACCTGTAC	259.4	143
D1Wox19	58-70	TGTAATGGAATCTGATGCCC GGGCTCTATAGATAGGAGGTTTTAT	185.7	160
D1Arb31	68	AGAATTAAGTGGGAGGCTGGGC AGAGGATAAAGGAAGGGCGTGG	64.2	294
D1Mco17 (Atp1a3)	66	TGAGCTTCTGGTTGAAGGATCG CTCCACATATACCACCAAAGGC	80.3	165
D1Rat1	60	GCAATGCCATGGGTTTACTC AAAAGTTATCCCCTTCCCCC	10.6	129
D1Rat15	62-60	TGGAATGAAGGGGCTTACTG GTACAGGATGGCACTCGGTT	37.8	157
D1Rat27	60	TCTCTCCAGCTGCAGGATTT GGGCAAGCAAAGTACATGGT	90.3	177
D1Rat173	60-58	GATGGAGGCAGTTTTTCCAA GATCCCTTGACAAGCATGGT	145.3	156
D1Rat259	62	GTGGAACAGAGGGACTGCTT GCTTCCCTTCTCTGTGTTGAA	77.15	203
D1Rat28	60	ATGCACTCTATGATTGGCCC TGTCAGGACACATTCCTGCT	88.9	148
D1Mgh9	62-66	TGACCTCCACACGTGCTAAG AGAATGCTCAGGAAAAGTTAGGG	176.9	140
D1Rat195	58-60	CCCAGCATCAACCTCTTCC TTAACCTGCTTGGTTTTGGG	264.8	210

The five primers selected for QTL analysis are marked in bold. Legend: Mb – megabases; PCR – polymerase chain reaction; b.p. – base pairs.