Table 1.
The primers used in the Real Time-PCR.
| Genes | GenBank accession number | Primers |
|---|---|---|
| MAT | NM_013283 | F: 5'- ACTTTGTTCCCGGGAGCTGTC -3' |
| R: 5'- AACTGCATGCCAATTATTCTGCTG -3' | ||
| SAHH | NM_000687 | F: 5'- CACCACAGGCTGTATTGACATCATC -3' |
| R: 5'- GTCCAATGTTACACACAATGGCATC -3' | ||
| MTR | NM_000254 | F: 5'-TAAGATTTGCAAAGGTTGGGTCTGA-3' |
| R: 5'-CTGGACATACAGGTGGGAGTTGG-3' | ||
| MTHFR | NM_005957 | F: 5'- TGTGTGAATTCTGCAACTAGCCAAG -3' |
| R: 5'- ATGAGCCACCACACCTGCTG -3' | ||
| CSE | NM_153742 | F: 5'-CCTTTGGCTCTGGGAGCTGATA-3' |
| R: 5'-ATTAACAGACACCAGGCCCATTACA-3' | ||
| CBS | NM_000071 | F: 5'-TGTGGGCACACCATCGAGA-3' |
| R: 5'-AGCGTCACCATTCCCAGGATTA-3' | ||
| β-actin | NM_001101 | F: 5'- CGACAACGGCTCCGGCATGT-3' |
| R: 5'- TGGGCCTCGTCGCCCACATA-3' |
MAT: methionine adenosyltransferase; SAHH: S-adenosylhomocysteine hydrolase; MTR: 5-methyltetrahydrofolate-homocysteine methyltransferase; MTHFR: 5, 10-methylenetetrahydrofolate reductase; CSE: cystathionine γ-lyase; CBS: cystathionine β-synthase; β-action: housekeeping gene