Table 1.
Macro parameters in MTP, Shake flask and Bioreactor
| Un-optimized MTP1 |
Optimized MTP2 |
SF2 | Bioreactor3 | |
|---|---|---|---|---|
| μmax (h-1) | 0.03 ± 0.008 | 0.11 ± 0.01 | 0.11 ± 0.01 | 0.10 ± 0.005 |
| Final Biomass (g/L) | 2.0 ± 0.13 | 4.0 ± 0.13 | 3.7 ± 1.3 | 4.1 ± 0.1 |
| UDP (mg/L) | 6.4 ± 1.6 | 80 ± 4.4 | 28 ± 15.5 | 65 ± 3.3 |
| ACT (mg/L) | 18.5.0 ± 7.5 | 520 ± 17.0 | 267 ± 23.6 | 500 ± 17.7 |
Biomass, UDP and ACT concentration indicated in the table corresponds to 120 hrs.
1Data based on 4 biological replicates each in triplicate. Un-optimized MTPs contained a medium with MES buffer and no glass beads.
2Data based on 3 biological replicates each in triplicate. Optimized MTPs and Shake flasks (SF) contained a medium with 100 mM MOPS buffer to maintain constant pH and 3 mm glass beads.
3Data based on three cultivations.