Figure 5.

Effect of lutein on PDGF-induced intracellular ROS production and H₂O₂ signaling. (A) VSMCs were treated with PDGF-BB (PDGF, 10 ng/ml) in the presence of vehicle (veh) or lutein (10 μM) for 10 min. Cells were immediately analyzed by fluorescence microscopy. The representative data from an experiment were shown (n = 5). The mean fluorescence intensity from the positive staining cells was calculated by the image analysis software. *P < 0.05 vs. control. (B and C) VSMCs were pretreated with vehicle (veh) or lutein for 30 min and followed by the addition of (B) H₂O₂ (50 μM) or (C) H₂O₂ (3 mM) for the indicated time. Cellular signaling was determined by Western blotting (n = 3). Quantitation of MAPKs and PDGFR and PLCγ phosphorylation was performed by densitometry. Data were expressed as (B) a bar graph or (C) indicated by a number.