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Immunoblots identifying 14-3-3 isoforms in extracts from mouse oocytes, eggs and ovaries. Proteins from cell lysates were separated by electrophoresis under reducing conditions, transferred to membranes and probed with antibodies directed against the 14-3-3 isoforms indicated. Each isoform shown was detected in lysates of 200 oocytes or 200 eggs. Protein extracts of ovaries and brain from adult mice are included for comparison. The 14-3-3 protein is approximately 30 kDa.
