Figure 2.

Comparison of the relative abundance of individual 14-3-3 isoforms in immature oocytes and mature eggs. The relative abundance of each individual isoform is based on the integrated densities of bands obtained from immunoblots (see Methods). Three experimental immunoblots were prepared for each of the six isoforms shown. In each experiment, for one of the isoforms, protein extracts from 200 oocytes and 200 eggs was analyzed after electrophoresis and immunoblotting on the same blot. For every blot, the density of the band for oocyte extracts was normalized to 100% and the density of the band for eggs is given as a percent with regards to the normalized oocyte value.