Table 3.
Bacterial strains and plasmids used in this study
| Strain/Plasmid | Description | Source |
|---|---|---|
| JM109 | E. coli K12 strain used for cloning | Promega |
| ATCC 31749 | Curdlan-producing Agrobacterium sp. (wild-type) | ATCC |
| ATCC 31749ΔnifR | ATCC 31749 mutant with gene knockout of nifR | This study |
| ATCC 31749Δppx1 | ATCC 31749 mutant with gene knockout of ppx1 (AGRO_2774) | This study |
| ATCC 31749Δppx2 | ATCC 31749 mutant with gene knockout of ppx2 (AGRO_2552) | This study |
| ATCC 31749ΔrpoN | ATCC 31749 mutant with gene knockout of rpoN | This study |
| ATCC 31749ΔrrpP | ATCC 31749 mutant with gene knockout of rrpP | This study |
| ATCC 31749ΔrelA/spoT | ATCC 31749 mutant with gene knockout of AGRO_1497 | This study |
| ATCC 31749Δ0033 | ATCC 31749 mutant with gene knockout of AGRO_0033 | This study |
| ATCC 31749Δ0636 | ATCC 31749 mutant with gene knockout of AGRO_0636 | This study |
| ATCC 31749Δ1729 | ATCC 31749 mutant with gene knockout of AGRO_1729 | This study |
| ATCC 31749Δ3967 | ATCC 31749 mutant with gene knockout of AGRO_3967 | This study |
| DH5α/pUCP30T | E. coli strain containing suicide vector | H. Schweitzer |
| pGEM-T easy | Vector used for cloning internal gene fragments | Promega |
| pUCP30T-nifR | Gene knockout plasmid for interruption of nifR | This study |
| pUCP30T-ppx1 | Gene knockout plasmid for interruption of ppx1 (AGRO_2774) | This study |
| pUCP30T-ppx2 | Gene knockout plasmid for interruption of ppx2 (AGRO_2552) | This study |
| pUCP30T-rpoN | Gene knockout plasmid for interruption of rpoN | This study |
| pUCP30T-rrpP | Gene knockout plasmid for interruption of rrpP | This study |
| pUCP30T-spoT | Gene knockout plasmid for interruption of spoT | This study |
| pUCP30T-0033 | Gene knockout plasmid for interruption of AGRO_0033 | This study |
| pUCP30T-0636 | Gene knockout plasmid for interruption of AGRO_0636 | This study |
| pUCP30T-1729 | Gene knockout plasmid for interruption of AGRO_1729 | This study |
| pUCP30T-3967 | Gene knockout plasmid for interruption of AGRO_3967 | This study |