Figure 3.

BBB permeability does not correlate with onset of atypical disease. Representative Evans blue infiltrated areas of the cerebella/brainstems and spinal cords of the various groups of mice at EAE onset. CNS areas showing Evans blue staining (see Additional file 2) were cut transversely and photographed using a Zeiss stereoscope. Two (out of 4-6) representative mice per group are shown. IFNγ-/- (A-D), IFNγRKO-chimeras (I-L) and IFNγR^periKO (U-X) exhibited substantial Evans blue staining both in cerebellum and spinal cord. Evans blue staining patterns in the WT (E-H), WT-chimeras (Q-T) and IFNγR^CNSKO (M-P) were meningeal and confined in small areas in the spinal cord and were rarely detected in the cerebellum/brainstem parenchyma. Numbers in pink and white denote atypical and classic neurological deficits, respectively.