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. Author manuscript; available in PMC: 2013 Feb 23.
Published in final edited form as: Neuron. 2012 Feb 23;73(4):713–728. doi: 10.1016/j.neuron.2011.12.021

Figure 2. Sip1 is Required for Oligodendrocyte Myelination.

Figure 2

(A) Schematic diagram show that excision of the floxed Sip1 exon 7, which encodes a majority of protein sequence, is mediated by Olig1-Cre.

(B) Sip1cKO (Sip1lox/lox;Olig1Cre+/−) mice developed tremors and hindlimb paralysis starting around P10. Upper panel, a control mouse (Ctrl, Sip1lox/+;Olig1Cre+/−) in comparison to a cKO sibling at P14. Lower panel, optic nerves from control and Sip1cKO littermates at P14.

(C) Survival curve of control and Sip1cKO mice, which died around postnatal week 3 (n ≥ 32).

(D-F) Expression of Mbp and Plp1 in the cortex (Ctx), spinal cord (SC) and cerebellum (CB) from control (Ctrl) and Sip1cKO mice at P14 by in situ hybridization. Arrows indicate MBP+ and Plp1+ cells in the white matter.

(G-H) Electron micrographs of the optic nerve (G) and spinal cord (H) of control and Sip1cKO mice at P14. High power images (right panels in G, H) showing that multilamellar myelin sheaths are apparent around many axons in control mice, whereas all axons in Sip1cKO mice are essentially unmyelinated.

Scale bars in D, F: 100 μm; G, H: left panels, 5 μm; right panels; 1 μm