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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Cancer Cell. 2011 Oct 18;20(4):538–549. doi: 10.1016/j.ccr.2011.08.025

Figure 6. VCAM-1 engagement by α4 integrins triggers anti-apoptotic signals via the cytoplasmic domain.

Figure 6

(A) VCAM-1 clustering antibodies (Ab X-linking) were added to the indicated cell lines in the presence or absence of 10 ng/ml TRAIL for 3 h. Caspase-3 activation was detected by western immunoblotting.

(B-D) The indicated cancer cells were co-cultured with U937 cells in the presence of TRAIL and α4 integrin antibody (anti-α4) or comparable Ig control (-). (B) After 24 h, TUNEL+ (red) and green CMFDA-labeled cancer cells were scored under fluorescent microscope. Arrow indicates a TUNEL+/CMFDA+ cancer cell and asterisk indicates a (CMFDA-negative) U937 cell. (C) Percentage of TUNEL+/CMFDA+ cancer cells. Data are average ± SEM; n = 10 unit areas; p value calculated using t test. Scale bar, 20 μm. (D) Cancer cells were quantified after 48 h of co-culture. Data are average ± SEM; n = 4 samples.