Table 1.

MAb EF13D binding mutations identified by random or site-directed mutagenesis

EF DIII MUTANT	BINDING	PARTIAL BINDING	LOSS OF BINDING
E662V		x
L667F			x
L667S			x
S668P			x
S669C	x
I670F			x
R671I			x
R672G			x
V676A	x
V678D		x
K680N	x
K690I	x
V694A		x
V694G		x
S693R	x
K695E	x
K696T		x
I697N			x
L701S			x
Y704C		x
Y705C	x
N709D	x
H710R	x
F712L	x
Q714R	x
S721P	x
Q727L	x
A728D			x
I732T		x
L736P			x
K737N	x
Y748N			x
Y748C			x
L762S	x
L763P		x
I771T	x
Q779L	x
N785K	x
V792D	x
I797T		x
E799G	x