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. 2011 Oct 22;80(1):89–100. doi: 10.3797/scipharm.1109-03

Tab. 2.

Validation parameters of the HPLC method for ANT, CBZ, FSD and PHTN

Parameter Value

ANT CBZ FSD PHTN
Analytical wavelength 230 nm 205 nm 230 nm 205 nm
Range (μM) 5–100 5–100 5–100 5–100
Slope (Mean ±S.D; R.S.D.) 7668.8 ± 103.3; 1.3 16075.5 ± 39.3; 0.24 22085.1 ± 149.0; 0.67 13577.6 ± 18.19; 0.13
Intercept (Mean ± S.D.) −5541.3 ± 172.8 −3746.8 ± 66.91 1426.1 ± 191.9 −3443.4 ± 386.1
Regression coefficient 0.999 1.000 0.999 1.000
LOD (μM) 3.28 0.67 0.13 0.73
LOQ (μM) 5.28 2.24 0.58 2.04

Each standard curve was generated in triplicate on 3 consecutive days, across the linearity range. Values are reported as mean±SD of three calibration curves.