Tab. 2.
Validation parameters of the HPLC method for ANT, CBZ, FSD and PHTN
Parameter | Value | |||
---|---|---|---|---|
|
||||
ANT | CBZ | FSD | PHTN | |
Analytical wavelength | 230 nm | 205 nm | 230 nm | 205 nm |
Range (μM) | 5–100 | 5–100 | 5–100 | 5–100 |
Slope (Mean ±S.D; R.S.D.) | 7668.8 ± 103.3; 1.3 | 16075.5 ± 39.3; 0.24 | 22085.1 ± 149.0; 0.67 | 13577.6 ± 18.19; 0.13 |
Intercept (Mean ± S.D.) | −5541.3 ± 172.8 | −3746.8 ± 66.91 | 1426.1 ± 191.9 | −3443.4 ± 386.1 |
Regression coefficient | 0.999 | 1.000 | 0.999 | 1.000 |
LOD (μM) | 3.28 | 0.67 | 0.13 | 0.73 |
LOQ (μM) | 5.28 | 2.24 | 0.58 | 2.04 |
Each standard curve was generated in triplicate on 3 consecutive days, across the linearity range. Values are reported as mean±SD of three calibration curves.