Tab. 5.
Antibacterial spectrum of activity of bacteriocins produced by selected strains.
| Bacteriocin Activity of Producer strains | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
||||||||||||||
| Indicator strains | Source | Growth conditions | 2S | 5S | 6S | 7S | 8S | 9S | 10S | 11S | 16S | 18S | 1K | 2K |
| lactic acid bacteria | ||||||||||||||
|
| ||||||||||||||
| Lb. plantarum 3S | Our isolate | MRSc,30°C | ++ | ++ | +++ | ++ | ++ | ++ | + | ++ | ++ | ++ | ++ | ++ |
| Lb. plantarum 9S | Our isolate | MRS,30°C | − | − | − | − | − | − | − | − | − | − | − | − |
| Lb. plantarum 29V | Our collection | MRS,30°C | − | − | − | − | − | − | − | − | − | − | − | − |
| Lb. rhamnosus 18S | Our isolate | MRS,30°C | − | − | − | − | − | − | − | − | − | − | − | − |
| Lb. rhamnosus 1K | Our isolate | MRS,30°C | − | − | − | − | − | − | − | − | − | − | − | − |
| Lb. fermentum 2K | Our isolate | MRS,30°C | − | − | − | − | − | − | − | − | − | − | − | − |
| Enterococcus faecium | DSMa 13596 | BHId,37°C | − | − | − | − | − | − | − | − | − | − | − | − |
|
| ||||||||||||||
| Gram-positive pathogenic bacteria | ||||||||||||||
|
| ||||||||||||||
| Staphylococcus aureus | ATCC 25923 | BHI, 37°C | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Staphylococcus aureus (MDR) | Clinical isolatef | BHI, 37°C | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Bacillus cereus | ATCC 11778 | BHI, 37°C | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Streptococcus mutans | DSM 20523 | BHI, 37°C | ++ | +++ | +++ | ++ | ++ | ++ | ++ | ++ | +++ | ++ | +++ | +++ |
|
| ||||||||||||||
| Gram-negative pathogenic bacteria | ||||||||||||||
|
| ||||||||||||||
| Escherichia coli | ATCC 11775 | BHI, 37°C | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Escherichia coli (MDR) | Clinical isolate | BHI, 37°C | − | − | + | − | − | − | − | − | − | − | ++ | − |
| Salmonella Typhi | ATCC 6539 | NBe, 37°C | +++ | +++ | +++ | ++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Pseudomonas aeruginosa | ATCC 9027 | BHI, 37°C | +++ | ++ | +++ | +++ | ++ | ++ | ++ | ++ | +++ | ++ | +++ | ++ |
| Pseudomonas aeruginosa | ATCC 27853 | BHI, 37°C | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
| Klebsiella pneumoniae | Clinical isolate | BHI, 37°C | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ | ++ |
| Shigella flexneri | Clinical isolate | NB, 37°C | + | + | ++ | ++ | ++ | +++ | ++ | ++ | +++ | ++ | ++ | +++ |
Inhibition zone Diameter: +++ = >14mm; ++ = 11–14mm; + = 8–10mm; − = no inhibition. MDR: Multidrug-resistant;
a:
DSM: Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany;
b:
ATCC: American Type Culture Collection, Manassas, VA, USA;
c:
de Man, Rogosa and Sharpe;
d:
Brain Heart Infusion;
e:
Nutrient Broth;
f:
Isolates from Centre Pasteur of Yaoundé, Cameroon.