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ERA-positive human mammary epithelial HME1 cells are endowed with an inner functional clock. Quantitative RT-PCR analysis on cultured HME1 cells at 4-h intervals after entrainment via serum shock shows that the mRNA of the clock genes PER1, PER2, PER3, CRY1, CRY2, RevErbA and BMAL1 accumulates in a circadian fashion, while the mRNA of CLOCK does not show any circadian oscillation.
