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Direct immunofluorescence with a 1 to 20 dilution of antibody to human IgG conjugated to fluorescein isothiocyanate (green fluorescence) shows IgG deposition at the junction of plaque-periplaque white matter (middle arrow), in mononuclear cells (bottom arrow), and in normal white matter (top arrow). The antigen against which the IgG in the multiple sclerosis brain and CSF is directed is unknown.
