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Streamlined protocol to determine specificity of phage peptides. Individual phage plaque from second and third pans amplified in U96 DeepWell plates are first screened for positivity against panning antibody using enzyme-linked immunosorbent assay (ELISA). Potential positive clones were confirmed by duplicate ELISA with preimmune human IgG/irrelevant rAb as negative control, followed by phage DNA purification and sequence analysis.
