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. 2012 Feb 7;3:662. doi: 10.1038/ncomms1648

Figure 5. Monomeric (MutM) mutants of Beclin 1 CC domain retain interaction with Atg14L and UVRAG.

Figure 5

(a,b) Interaction of Beclin 1 wild-type and monomeric (MutM) mutants with Atg14L or UVRAG in cultured cells. HEK 293T cells were transfected with GFP-tagged Beclin 1 variant alone (a) or together with FLAG-UVRAG (b). The lysates were immunoprecipitated by GFP antibody, followed by western-blot analysis with anti-Atg14L antibody for detecting binding to endogenous Atg14L (a) or with anti-UVRAG antibody for detecting binding to the FLAG-tagged UVRAG protein (b).