Table 1. Summary of the binding affinity (Kd) and stoichiometry of for Beclin1-Atg14L/UVRAG interaction as measured by ITC and analytic ultracentrifugation experiments.
| Beclin CCD | Kd (Atg14L) | Kd (UVRAG) | Mr (kDa) (w/Atg14L) | Mr (kDa) (w/UVRAG) |
|---|---|---|---|---|
| WT | 3.22±0.95 μM | 0.24±0.06 μM | 22.3 (Heterodimer) | 18.5 (Heterodimer) |
| L178A/L192A | 4.61±0.50 μM | 2.24±0.24 μM | 21.9 (Heterodimer) | 19.1 (Heterodimer) |
| L178A/L196A | 6.85±0.84 μM | 1.14±0.13 μM | 22.6 (Heterodimer) | 18.4 (Heterodimer) |
| L178A/L259A | 1.20±0.13 μM | 0.07±0.01 μM | 23.5 (Heterodimer) | 19.5 (Heterodimer) |
| E189L | No | 1.13±0.14 μM | — | — |
| R203L | No | No | — | — |
| E224L | 8.85±0.40 μM | 2.43±0.47 μM | — | — |
| E189L/A255L | No | ∼1.31 μM (est.) | — | — |
| A217L/E224L | No | 1.44±0.52 μM | — | — |
| A217L/E224L/A255L | No | 4.24±0.51 μM | — | — |
| E189L/A217L/E224L/A255L |
No |
8.70±3.03 μM |
— |
— |
Abbreviation: WT, wild type.