Fig. 1.

Inhibition cell migration of SK-MEL-28 (A) and T24 (B) cells were measured when r-mojastin 1 (5.3 µM) and r-mojastin-GST (5.3 µM) were added to tissue culture media. A confluent monolayer of cells was maintained in medium, and a line was scraped through the monolayer of cells with a plastic, sterile pipette tip. The cultures were allowed to migrate for 24 h at 37 °C in the presence or absence of recombinant disintegrins. The extent of wound closure was quantified by multiple measurements of the width of the scrape space for each cell line.