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A. Analysis of proximal region of the SCN5a promoter showed two overlapping conservative Foxo binding insulin response elements, 5′-CAAAACA-3′, in rat, mouse and human. B. ChIP assay showed that Foxo1 bound SCN5a promoter region in both HL-1 cells (upper panel) and adult mouse ventricular myocytes (lower panel) using two sets of paired primers, respectively. Lane 1 and 2 were input and IgG, respectively.
