TABLE 3.

Summary of NTD cognate and non-cognate TRS association binding parameters

Conditions were 50 mm KP_i, 100 mm KCl, pH = 6.0 at 25.0 °C. K_obs values are averaged values from three independent fluorescence anisotropy titrations. ND, not determined. Errors report the S.D. of the triplicate measurements.

Protein	F-TRSa	Kobs (× 107m−1)	r0	rcomplex
MHV NTD WT	MHV	4.3 ± 0.4	0.063	0.212
	IBV	1.25 ± 0.05	0.072	0.211
	SARS-CoV	0.306 ± 0.0005	0.064	0.133
	Scrambled	1.70 ± 0.06	0.062	0.198
MHV NTD R110A	MHVb	4.1 ± 0.2	0.045	0.172
	IBV	0.66 ± 0.09	0.054	0.190
MHV NTD Y129A	MHVb	3.5 ± 0.3	0.054	0.190
	IBV	0.9 ± 0.2	0.072	0.195
IBV NTD WT	IBV	0.084 ± 0.001	0.070	0.170
	MHV	0.159 ± 0.02	0.068	0.211
	Scrambled	0.179 ± 0.03	0.0622	0.176
IBV NTD R76A	IBV	0.0095 ± 0.0007	0.072	0.174
IBV NTD Y94A	IBV	0.0453 ± 0.0004	0.068	0.174
SARS-CoV NTD WT	SARS-CoV	0.277 ± 0.0009	0.061	0.133
	MHV	1.2 ± 0.03	0.069	0.171
	Scrambled	0.81 ± 0.03	0.063	0.134

^a RNA sequences are given in Table 2 (see also supplemental Fig. S9).

^b Reported in Ref. 18.