FIGURE 3.

DhPPZ1 knock-out affects growth of D. hansenii. A, growth curve of dhppz1 mutant (DBH936) and control parental (DBH93) D. hansenii strains are shown. Saturated cultures DBH936 and DBH93 were re-inoculated 100 ml of YPD medium at initial A₆₀₀ of 0.05. Growth of these cultures was monitored by measuring A₆₀₀ at regular intervals. B, shown is reversal of slow growth phenotype of dhppz1 mutant by expressing DhPPZ1 from plasmid. Saturated cultures of DBH936 strains transformed with indicated plasmids were re-inoculated into 25 ml of SD medium (at initial A₆₀₀of 0.025). After 24 h of growth at 28 °C, A₆₀₀ of these cultures was measured. DBH93 strain transformed with plasmids pDA1 and pDH4 was used as control. Data were expressed as their relative growth with respect to the control. C, relative growth of dhppz1 mutant at different pH values is shown. Saturated cultures of DBH936 and DBH93 (control) were re-inoculated (initial A₆₀₀ 0.025) into 50 ml of YPD medium buffered to different pH values. After 24 h of growth at 28 ºC, A₆₀₀ of the culture was measured. The growth at pH 6.0 for the respective culture was taken 100%. Data presented are the results of three independent experiments (mean ± S.D.).