FIGURE 5.

Structure-function analysis of N-terminal domain of DhPpz1p. Schematics depicting wild type and mutant DhPpz1p are shown. C-terminal catalytic domain (light box), N-terminal non-catalytic domain (dark box), and the position of different deletions are shown. The right panel shows phenotypic complementation of dhppz1 mutation in DBH936 by different plasmid constructs as determined by dilution spotting on SD plates containing LiCl, hygromycin (Hyg), and caffeine (Caf). + indicates complementation, and − indicates no complementation. Experiments were repeated three times with similar results.