TABLE 1.
Strains and plasmids used in this study
| Strain/Plasmid | Descriptions | Source/Reference |
|---|---|---|
| Strain | ||
| D. hansenii | ||
| DBH 9 | dhhis4 | Ref. 30 |
| DBH93 | dhhis4 dharg1 | This study |
| DBH91 | dhhis4 dhppz1::DhHIS4 | This study |
| DBH936 | dhhis4 dharg1 dhppz1::DhHIS4 | This study |
| DBH932 | dhhis4 dharg1 dhmpk1::DhHIS4 | This study |
| S. cerevisiae | ||
| BY4742 | MAT α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 | Euroscarf |
| Y10557 | MAT α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0; ppz1::kanMX4 | Euroscarf |
| EGY 48 | MATα; trp1; his3; ura3; 6ops-LEU2 | Ref. 33 |
| Plasmids | ||
| pDH4 | DhHIS4 CfARS16 | Ref. 30 |
| pDH11 | DhHIS4 CfARS16 RFP (under DhTEF promoter) | Ref. 30 |
| pAN4 | DhHIS4 CfARS16 DhPPZ1-RFP | This study |
| pAN5 | DhARG1 CfARS16 DhPPZ1 | This study |
| pAN6 | DhARG1 CfARS16 DhPPZ1 (under DhTEF promoter) | This study |
| pAN51 | DhARG1 CfARS16 DhPPZ1 (Δ27–36) | This study |
| pAN52 | DhARG1 CfARS16 DhPPZ1 (Δ59–75) | This study |
| pAN53 | DhARG1 CfARS16 DhPPZ1 (Δ81–104) | This study |
| pAN54 | DhARG1 CfARS16 DhPPZ1 (Δ106–120) | This study |
| pAN55 | DhARG1 CfARS16 DhPPZ1 (Δ162–183) | This study |
| pAN56 | DhARG1 CfARS16 DhPPZ1 (Δ241–254) | This study |
| pAN9 | DhARG1 CfARS16 DhPPZ1 (Δ279–572) | This study |
| pAN10 | DhARG1 CfARS16 DhPPZ1 (Δ1–278) | This study |
| pDA1 | DhARG1 CfARS16 | This study |
| pRS-PPZ1 | HIS3 2μ PPZ1 | This study |
| PPZ1 Δ43–52 | HIS3 2μ PPZ1(Δ43–52) | This study |
| pEG202- DhPPZ1 | HIS3 2μ DhPPZ1 | This study |
| pEG202- DhPPZ1Δ27–36 | HIS3 2μ DhPPZ1(Δ27–36) | This study |
| pJG4-5-DhHAL3 | TRP1 2μ DhHAL3 | This study |
| pEG202 (bait vector) | HIS3 2μ | Ref. 33 |
| pJG4-5 (prey vector) | TRP1 2μ | Ref. 33 |