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Glutathione can activate TMX in vitro. Samples with ricin (2 μg) were incubated for 60 min at 37 °C in the absence (lanes 1 and 2) or in the presence (lanes 3 and 4) of 5 μg of oxidized TMX (final volume of 50 μl). GSH (1 mm) was present in samples of lanes 2 and 3. Samples with ricin alone (lane 1) or without TMX only (lane 2) were included as controls. The reduction of ricin was evaluated by Western blotting as described.
