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. 2012 Jan 6;287(10):7367–7373. doi: 10.1074/jbc.M111.316828

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — PDI and TMX expression and reduction of ricin in human cell lines. A, immunoblot identification of TMX and PDI in human cell lines extracts. Lane 1, A549 (respiratory epithelia); lane 2, DU145 (prostate cancer); lane 3, LNCaP (prostate cancer); lane 4, T3M4 (pancreatic cancer); lane 5, GER (pancreatic cancer); lane 6, PACA44 (pancreatic cancer); lane 7, MiaPaCa (pancreatic cancer); lane 8, CFPAC-1 (pancreatic cancer); lane 9, PT45 (pancreatic cancer); lane 10, Jurkat (T cell leukemia); lane 11, THP-1 (human monocyte). Human β-actin was used as a control to determine the relative amount of protein for each cell line. B, A549, DU145, PT45, and CFPAC-1 cells were incubated with ricin at various concentrations. The vitality was tested after 48 h by XTT assay as described above.