FIGURE 8.

RB enables site-specific methylation at the U6-1 locus. RB expression in Saos2 cells is correlated with increased CpG methylation. RB expression was induced for 48 h in Saos2-Tet-RB cells, and genomic DNA was harvested for bisulfite conversion and sequencing, as described previously. DNA sequences were analyzed for methylated cytosines using BISMA (36), and the frequency of methylation at each position is graphically represented. The number of clones sequenced is denoted by n. The dotted line denotes the level of methylation necessary to reach statistical significance of p = 0.05.