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. 2011 Dec 23;287(10):7313–7323. doi: 10.1074/jbc.M111.331660

FIGURE 4.

FIGURE 4.

Impact of PRMT5 on TPA-dependent hINV promoter activity. A, KERn were electroporated with 3 μg of control- or PRMT5-pooled (Santa Cruz) siRNA. After 48 h cells were re-electroporated with 3 μg of pINV-2473 and involucrin promoter luciferase reporter construct. After an additional 6 h, TPA was added, and after an additional 18 h cells were lysed for luciferase activity assay. The values are the mean ± S.E., n = 3. The asterisks indicate a significant difference as determined by Student's t test, p < 0.001, between control-siRNA and PRMT5-siRNA at 5 and 10 ng/ml TPA. B, KERn were electroporated with 3 μg of pcDNA3 or pcDNA-PRMT5 and after 48 h re-electroporated with 3 μg of pINV-2473. After an additional 6 h the cells were treated with TPA for 18 h, and then extracts were prepared for luciferase activity assay. The results are presented as the mean ± S.E., n = 3. The asterisks indicate a significant difference as determined by Student's t test, p < 0.001, between pcDNA3 and pcDNA3-PRMT5 at 5 and 10 ng/ml TPA.