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. 2011 Dec 23;287(10):7313–7323. doi: 10.1074/jbc.M111.331660

FIGURE 5.

FIGURE 5.

PKCδ regulates PRMT5 level. A, KERns were electroporated with 3 μg of control- and PKCδ-pooled (P, Santa Cruz) or individual (PKCδ-1, PKCδ-2) (40) siRNA, and after 24 h, PKCδ and PRMT5 mRNA levels were monitored as outlined in Fig. 2. B KERns were electroporated with 3 μg of control- or PKCδ-pooled (Santa Cruz) siRNA, and after 24 h extracts were prepared for detection of PKCδ, PRMT5, and SDMA by immunoblot. The values are the mean ± S.D. of three replicates, and similar results were observed in each of three experiments. C and D, KERn were infected with the indicated adenovirus, and after 24 h mRNA and protein extracts were prepared for detection of PKCδ and PMRT5 RNA and protein. SDMA was also monitored by immunoblot. The arrows in the SDMA blot indicate the major SDMA-modified proteins. The bar graph values are the mean ± S.D. of three replicated samples. Similar results were observed in each of three experiments.