FIGURE 1.

GPx4 expression in the wild-type mouse retina. A, lysates of the retina, retinal pigment epithelium (RPE)/choroid, and brain were immunoblotted for GPx4 and β-actin. GPx4 protein level in the retina was higher than that in RPE/choroid and brain. B–E, immunohistochemistry of the retina for GPx4 (green) at P1 and 8-week-old mice. GPx4 was abundantly expressed in the retina, especially in the inner segments (IS) of photoreceptor cells while there was no detectable expression of GPx4 protein in the outer segments (OS) depicted by autofluorescence. B and D, negative controls. GCL (ganglion cell layer), INL (inner nuclear layer), ONL (outer nuclear layer), NL (neuroblastic layer). Scale bar, 50 μm.