FIGURE 7.

Decreased mitochondrial biomass in photoreceptor cells of the CKO mice at P11. A–C, in IS of photoreceptor cells of the CKO mice, mitochondrial area on TEM was significantly smaller than in IS of the control mice (mean ± S.E.; n = 4; ***, p < 0.001 by two-tailed t test). D, in the whole retina, mitochondrial DNA (mtDNA) replication was decreased in the CKO mice in comparison to the control mice (mean ± S.E.; n = 3; *, p < 0.05, by two-tailed t test). E, a paradoxical increase in Tfam mRNA in the whole retina of the CKO mice, suggesting a compensatory aspect of Tfam against the mitochondrial decrease by GPx4 deficit (mean ± S.E.; n = 3; **, p < 0.01 by two-tailed t test). Scale bar, 1 μm.