FIGURE 1.
Oma1 specifically mediates Cox1 degradation in coa2Δ cells. A, respiratory growth of Coa2-deficient cells lacking various proteases. Cells were pregrown in complete liquid medium, serially diluted, and spotted onto plates containing 2% glucose or glycerol/lactate as a carbon source. B, in vivo labeling of mitochondrial translation products in the cells described in A. The cells were pulsed for 15 min with [35S]methionine at 30 °C. The reaction was stopped by addition of cold methionine. Following a 60-min chase at 30 °C, samples were subjected to 12% SDS-PAGE and analyzed by autoradiography. C, respiratory growth of the shy1Δ and coa1Δ strains with and without Oma1. Cells were handled as described in A. Cyt. b, cytochrome b.