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SDS-PAGE (silver stain) analysis of CAPs. Peptides (50 ng) were diluted in 1× SDS-containing sample buffer and incubated for 30 min at room temperature prior to electrophoresis using NuPAGE 12% BisTris gel at 200 V. MWexp/MWtheor is the ratio of experimentally determined to actual molecular weight of each CAP, where a value of ∼2 corresponds to the dimer. Molecular mass standards are given to the right of the gel. The molecular weights of CAPs are given in Table 1.
