TABLE 2.
Effect of IVM on peak current and deactivation
Deactivation rates are the t90–10% times measured from the falling phase of the ligand-gated current that occured upon washout of ATP. ATP was applied at a concentration of 100 μm for zebrafish experiments and at a concentration of 3 μm in all other experiments. The concentration of IVM was 3 μm. The number of trials for each experiment is indicated in parentheses.
| Protein | -Fold change in peak current by IVM | Deactivation rate control | Deactivation rate IVM | -Fold change in deactivation |
|---|---|---|---|---|
| s | s | |||
| hP2X4R | 4.2 ± 0.9 (15) | 0.14 ± 0.01 (18) | 17.4 ± 2.4 (14) | 124.3 |
| hP2X4R-Y42W | 1.2 ± 0.1 (10) | 7.9 ± 1.1 (17) | 14.2 ± 2.7 (9) | 1.8 |
| hP2X4R-V43W | 0.6 ± 0.1 (8) | 3.0 ± 0.4 (9) | 19 ± 3.3 (5) | 6.3 |
| hP2X4R-E51Q | 2.4 ± 0.5 (8) | 0.32 ± 0.03 (11) | 3.9 ± 1.1 (7) | 12.2 |
| hP2X4R-D331S | 6.9 ± 1.5 (5) | 0.36 ± 0.05 (13) | 27.6 ± 6.4 (5) | 76.7 |
| hP2X4R-E51Q/D331S | 2.2 ± 0.4 (6) | 0.13 ± 0.01 (11) | 1.7 ± 0.4 (5) | 13.1 |
| zfP2X4R | 8.7 ± 3.5 (5) | 0.13 ± 0.05 (4) | 0.6 ± 0.1 (4) | 4.6 |
| zfP2X4R-N54E/N334D | 40.1 ± 11.0 (4) | 0.4 ± 0.1 (4) | 14.0 ± 2.5 (4) | 35 |