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Purification of SERCA2a cross-linked to PLB using anti-PLB monoclonal antibody affinity chromatography. SERCA2a purified as indicated in Fig. 3 (Input) was subjected to anti-PLB monoclonal antibody affinity chromatography. A, Coomassie Blue stained gel of selected fractions. B and C, immunoblots of the same fractions probed with 2A7-A1 and 2D12, respectively.
