FIGURE 2.

PKCζ is required for angiotensin II-mediated stimulation of the ERK5 pathway in cardiac fibroblasts. A, neonatal mouse cardiac fibroblasts were challenged with angiotensin (AngII), and ERK5 stimulation was determined as described in Fig. 1B. Data (mean ± S.E. of three independent experiments) were normalized using actin as a loading control and expressed as fold induction compared with the absence of agonist. *, p < 0.05; **, p < 0.01. B and C, mouse (B) or rat (C) cardiac fibroblasts were preincubated with vehicle or with a myristoylated PKCζ pseudosubstrate (PS) inhibitor (10 μm) as described under “Experimental Procedures” challenged with 100 nm angiotensin for the indicated times, and ERK5 activation was determined as described in A. Blots are representative of two to three experiments.