FIGURE 3.

Activation of MEK5 and MEF-2C upon chronic angiotensin administration is inhibited in PKCζ-deficient mice. PKCζ-deficient mice (PKCζ^−/−) and matched WT control littermates were subjected to chronic angiotensin II (AngII) infusion (or PBS, as a control vehicle) for 14 days using osmotic minipumps as detailed under “Experimental Procedures.” The activation status of MEK5 and MEF2C was assessed by immunohistochemistry using phosphospecific antibodies. Brown shading indicates positive DAB staining. Signal intensity was quantified as detailed under “Experimental Procedures,” and data plotted below represent the average of three independent experiments. **, p < 0.01; ***, p < 0.001, two-tailed t test when compared with control with angiotensin II-treated wild-type mice. a.u., arbitrary units.