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. 2012 Jan 17;287(10):7766–7779. doi: 10.1074/jbc.M111.290718

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DFF40/CAD is not detected in the chromatin-enriched fraction of SK-N-AS cells upon STP treatment. A, SK-N-AS (SK) and SH-SY5Y (SH) cells were treated (+) or not (−) with 1 μm STP for 6 h. After that, we performed the biochemical fractionations as described in Fig. 5A. From the nuclear (N in Fig. 5A) fraction obtained, we further separated the chromatin-enriched (N2) fraction from the nucleoplasmic (N1) fraction, and DFF40/CAD protein was analyzed by Western blot (upper panel). PKM2 (middle panel) and topoisomerase I (TOPO I, lower panel) were run as cytosolic and nuclear protein markers, respectively. B, genomic DNA analysis of the different fractions obtained in A was achieved by conventional agarose gel electrophoresis and subsequent ethidium bromide staining. Note that DNA is mainly observed in the N2 chromatin-enriched fraction in both cell lines.