DFF40/CAD detected in the chromatin-enriched fraction of STP-treated SH-SY5Y cells comes from the cytosolic pool of the endonuclease.
A, SH-SY5Y cells were treated with different concentrations of STP (0, 0.25, 0.5, 1, 2, 4, 8 μm) for 6 h, and then cells were fractionated to obtain the chromatin-enriched or N2 (A), the cytosolic or C (B), and the nucleoplasmic or N1 (C) fractions. DFF40/CAD protein detection was performed by Western blot in the different fractions obtained. PKM2 (middle panel) and topoisomerase I (TOPO I, lower panel) were run as cytosolic and nuclear protein markers, respectively.