FIGURE 3.

Suppression of SKIP did not affect insulin-induced insulin receptor and Erk1/2 activation. C2C12 cells transfected with specific siRNAs were stimulated with insulin (0–100 nm) for 10 min. Insulin-induced tyrosine phosphorylation of Erk1/2 (A) and insulin receptor β (B) was determined by Western blot analysis and quantified by densitometry. All values are presented as mean ± S.E. (error bars).