FIGURE 1.

Down-regulation of CD8-DMα reporter molecules by MARCH8. CD8-DM reporters were transiently expressed in HEK-293T cells together with EGFP-MARCH8, EGFP-MARCH8mut, or pEGFP. Cell surface expression of CD8 was measured by FACS using anti-CD8 mAb OKT8 and PE anti-mouse mAb (FL2). A, dot plots showing levels of CD8-DMα (upper) and CD8-DMα-K230R (lower) in cells co-expressing EGFP-MARCH8, EGFP-MARCH8 mutant, or pEGFP. B, histograms showing surface levels of CD8-DMα (left) and CD8-DMα-K230R (right) in cells expressing EGFP-MARCH8, as determined by EGFP expression using gates R4–6 of A. Data are representative of at least three independent experiments.