TABLE 1.
Amino acid composition of constructs used in this study
Numbering of residues is derived from mature proteins after signal peptide cleavage. Substituted residues are in boldface type.
| Construct | Extracellular domain | Trans-membrane | Cytoplasmic tail |
|---|---|---|---|
| CD8-DMα | CD8α | DMα | RKPCSGD |
| CD8-DMα-K230R | CD8α | DMα | RRPCSGD |
| DMα | DMα | DMα | RKPCSGD |
| DMα-K230R | DMα | DMα | RRPCSGD |
| DMα-K230R/C2132G/S233A (KCS-RGA) | DMα | DMα | RRPGAGD |
| DMβ | DMβ | DMβ | RRAGHSSYTPLPGSNYSEGWHIS |
| DMβ-Y230A | DMβ | DMβ | RRAGHSSATPLPGSNYSEGWHIS |
| DMβ-Δ224 | DMβ | DMβ | RR |
| DMβ-Y230F | DMβ | DMβ | RRAGHSSFTPLPGSNYSEGWHIS |
| DMβ-ST-A | DMβ | DMβ | RRAGHAAYAPLPGANYAEGWHIA |
| DMβ-HA | DMβ | DMβ | RRAGHSSYTPLPGSNYSEGWHISYPYDVPDYA |