FIGURE 1.

RDCs for ¹⁵N-labeled thymine bases of the W-box DNA. a, sequence of the double-stranded DNA used in the present NMR analysis. The W-box core sequence is boxed. The underlined T bases were ¹⁵N-labeled for the RDC analysis. b, IPAP-HSQC spectra of the complex between AtWRKY4-C and the W-box DNA containing the ¹⁵N-labeled T bases with (right panel) or without (left panel) 12 mg/ml Pf1 phage. The upfield and downfield components are shown in black and gray, respectively, and the coupling values are indicated in Hz. The RDCs are shown below the coupling values in the right panel.